AF2045

antibody from R&D Systems

Targeting: LGALS9 LGALS9A

Provider product page for AF2045

Western blot

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [11]
Comments [0]
Validations
Western blot [2]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: AF2045 - Provider product page
Provider: R&D Systems
Product name: Human Galectin-9 Antibody
Antibody type: Polyclonal
Description: Antigen Affinity-purified. Detects human Galectin-9 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Galectin-3 and rhGalectin-4 is observed and less than 2% cross-reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-7, and rhGalectin-8 is observed.
Reactivity: Human
Host: Goat
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 ug
Concentration: LYOPH
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

LGALS9 protein structure - AF2045 shown in red.

Submitted references Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen.

Schroeder JT, Bieneman AP
Journal of immunology (Baltimore, Md. : 1950) 2017 Aug 1;199(3):855-865

Tim-3 Is Upregulated in NK Cells during Early Pregnancy and Inhibits NK Cytotoxicity toward Trophoblast in Galectin-9 Dependent Pathway.

Sun J, Yang M, Ban Y, Gao W, Song B, Wang Y, Zhang Y, Shao Q, Kong B, Qu X
PloS one 2016;11(1):e0147186

Galectin-1, -3 and -9 Expression and Clinical Significance in Squamous Cervical Cancer.

Punt S, Thijssen VL, Vrolijk J, de Kroon CD, Gorter A, Jordanova ES
PloS one 2015;10(6):e0129119

Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells.

Leskela S, Serrano A, de la Fuente H, Rodríguez-Muñoz A, Ramos-Levi A, Sampedro-Nuñez M, Sánchez-Madrid F, González-Amaro R, Marazuela M
PloS one 2015;10(4):e0123938

Galectin expression profiling identifies galectin-1 and Galectin-9Δ5 as prognostic factors in stage I/II non-small cell lung cancer.

Schulkens IA, Heusschen R, van den Boogaart V, van Suylen RJ, Dingemans AM, Griffioen AW, Thijssen VL
PloS one 2014;9(9):e107988

Human cytomegalovirus upregulates expression of the lectin galectin 9 via induction of beta interferon.

McSharry BP, Forbes SK, Cao JZ, Avdic S, Machala EA, Gottlieb DJ, Abendroth A, Slobedman B
Journal of virology 2014 Sep;88(18):10990-4

TIM-3 does not act as a receptor for galectin-9.

Leitner J, Rieger A, Pickl WF, Zlabinger G, Grabmeier-Pfistershammer K, Steinberger P
PLoS pathogens 2013 Mar;9(3):e1003253

Extended co-expression of inhibitory receptors by human CD8 T-cells depending on differentiation, antigen-specificity and anatomical localization.

Baitsch L, Legat A, Barba L, Fuertes Marraco SA, Rivals JP, Baumgaertner P, Christiansen-Jucht C, Bouzourene H, Rimoldi D, Pircher H, Rufer N, Matter M, Michielin O, Speiser DE
PloS one 2012;7(2):e30852

Upregulation of the Tim-3/galectin-9 pathway of T cell exhaustion in chronic hepatitis B virus infection.

Nebbia G, Peppa D, Schurich A, Khanna P, Singh HD, Cheng Y, Rosenberg W, Dusheiko G, Gilson R, ChinAleong J, Kennedy P, Maini MK
PloS one 2012;7(10):e47648

A crucial role for Kupffer cell-derived galectin-9 in regulation of T cell immunity in hepatitis C infection.

Mengshol JA, Golden-Mason L, Arikawa T, Smith M, Niki T, McWilliams R, Randall JA, McMahan R, Zimmerman MA, Rangachari M, Dobrinskikh E, Busson P, Polyak SJ, Hirashima M, Rosen HR
PloS one 2010 Mar 4;5(3):e9504

The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells.

Thijssen VL, Hulsmans S, Griffioen AW
The American journal of pathology 2008 Feb;172(2):545-53

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Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human Galectin-9 by Western Blot. Western blot shows conditioned media from HT-29 human colon adenocarcinoma cell line, human peripheral blood lymphocytes (PBL), and human dendritic cells untreated (-) or treated (+) with 10 µg/mL PHA for 6 days. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Galectin-9 at approximately 36 and 40 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human Galectin-9 by Western Blot. Western blot shows conditioned media from HT-29 human colon adenocarcinoma cell line, human peripheral blood lymphocytes (PBL), and human dendritic cells untreated (-) or treated (+) with 10 µg/mL PHA for 6 days. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Galectin-9 at approximately 36 and 40 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Galectin-9 in Human PBMCs. Galectin-9 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with Calcium Ionomycin and PMA using Goat Anti-Human Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Galectin-9 in Human Colon. Galectin-9 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.