Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-32474 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cdc45L Recombinant Rabbit Monoclonal Antibody (JJ091-04)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JJ091-04
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Cdc45L Recombinant Rabbit Monoclonal Antibody (JJ091-04)(Product # MA5-32474) and a 60 kDa band corresponding to Cdc45L was observed in the cell lines and tissues tested except Mouse Liver and Mouse spleen which are reported to be negative and also decreased upon PMA treatment in K-562. Modified whole cell extracts (1%SDS) of K-562 (Lane 1), K-562 treated with 50 ng/mL of PMA for 28hr (Lane 2), U-2 OS (Lane 3), Jurkat (Lane 4), MCF7 (Lane5), Mouse Testis (Lane 6), Mouse Thymus (Lane 7), Mouse Liver (Lane 8) and Mouse Spleen (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Cdc45L in 293T cells using a Cdc45L Monoclonal antibody (Product # MA5-32474) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Cdc45L in A549 cells using a Cdc45L Monoclonal antibody (Product # MA5-32474) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Cdc45L in Hela cells using a Cdc45L Monoclonal antibody (Product # MA5-32474) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Cdc45L of paraffin-embedded Human kidney tissue using a Cdc45L Monoclonal antibody (Product #MA5-32474). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Cdc45L of paraffin-embedded Mouse colon tissue using a Cdc45L Monoclonal antibody (Product #MA5-32474). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Cdc45L of paraffin-embedded Mouse kidney tissue using a Cdc45L Monoclonal antibody (Product #MA5-32474). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of Cdc45L in 293 cells using a Cdc45L Monoclonal Antibody (Product # MA5-32474) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.