Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- bs-3425R - Provider product page
- Provider
- Bioss
- Proper citation
- Bioss Cat#bs-3425R, RRID:AB_10857310
- Product name
- Smad3 (Ser423 + Ser425) Antibody
- Antibody type
- Polyclonal
- Description
- KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 [CS(p-S)V(p-S)]
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1ug/ul
- Storage
- Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Submitted references Brainstem cholinergic pathways diminish cardiovascular and neuroinflammatory actions of endotoxemia in rats: Role of NFκB/α7/α4β2AChRs signaling.
Caloric restriction reduces basal cell proliferation and results in the deterioration of neuroepithelial regeneration following olfactotoxic mucosal damage in mouse olfactory mucosa.
Comparing Effects of Transforming Growth Factor β1 on Microglia From Rat and Mouse: Transcriptional Profiles and Potassium Channels.
The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway.
Melatonin attenuated brain death tissue extract-induced cardiac damage by suppressing DAMP signaling.
Prognostic implication of NOTCH1 in early stage oral squamous cell cancer with occult metastases.
Berberine, an isoquinoline alkaloid suppresses TXNIP mediated NLRP3 inflammasome activation in MSU crystal stimulated RAW 264.7 macrophages through the upregulation of Nrf2 transcription factor and alleviates MSU crystal induced inflammation in rats.
A conditionally replicating adenovirus expressing IL-24 acts synergistically with temozolomide to enhance apoptosis in melanoma cells in vitro.
Interleukin-18 modulation in autism spectrum disorders.
Sallam MY, El-Gowilly SM, Fouda MA, Abd-Alhaseeb MM, El-Mas MM
Neuropharmacology 2019 Oct;157:107683
Neuropharmacology 2019 Oct;157:107683
Caloric restriction reduces basal cell proliferation and results in the deterioration of neuroepithelial regeneration following olfactotoxic mucosal damage in mouse olfactory mucosa.
Iwamura H, Kondo K, Kikuta S, Nishijima H, Kagoya R, Suzukawa K, Ando M, Fujimoto C, Toma-Hirano M, Yamasoba T
Cell and tissue research 2019 Nov;378(2):175-193
Cell and tissue research 2019 Nov;378(2):175-193
Comparing Effects of Transforming Growth Factor β1 on Microglia From Rat and Mouse: Transcriptional Profiles and Potassium Channels.
Lively S, Lam D, Wong R, Schlichter LC
Frontiers in cellular neuroscience 2018;12:115
Frontiers in cellular neuroscience 2018;12:115
The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway.
Zhan FL, Liu XY, Wang XB
Journal of cellular biochemistry 2018 Jan;119(1):536-546
Journal of cellular biochemistry 2018 Jan;119(1):536-546
Melatonin attenuated brain death tissue extract-induced cardiac damage by suppressing DAMP signaling.
Sung PH, Lee FY, Lin LC, Chen KH, Lin HS, Shao PL, Li YC, Chen YL, Lin KC, Yuen CM, Chang HW, Lee MS, Yip HK
Oncotarget 2018 Jan 9;9(3):3531-3548
Oncotarget 2018 Jan 9;9(3):3531-3548
Prognostic implication of NOTCH1 in early stage oral squamous cell cancer with occult metastases.
Wang S, Fan H, Xu J, Zhao E
Clinical oral investigations 2018 Apr;22(3):1131-1138
Clinical oral investigations 2018 Apr;22(3):1131-1138
Berberine, an isoquinoline alkaloid suppresses TXNIP mediated NLRP3 inflammasome activation in MSU crystal stimulated RAW 264.7 macrophages through the upregulation of Nrf2 transcription factor and alleviates MSU crystal induced inflammation in rats.
Dinesh P, Rasool M
International immunopharmacology 2017 Mar;44:26-37
International immunopharmacology 2017 Mar;44:26-37
A conditionally replicating adenovirus expressing IL-24 acts synergistically with temozolomide to enhance apoptosis in melanoma cells in vitro.
Liang Z, Yang CS, Gu F, Zhang LS
Oncology letters 2017 Jun;13(6):4185-4189
Oncology letters 2017 Jun;13(6):4185-4189
Interleukin-18 modulation in autism spectrum disorders.
Businaro R, Corsi M, Azzara G, Di Raimo T, Laviola G, Romano E, Ricci L, Maccarrone M, Aronica E, Fuso A, Ricci S
Journal of neuroinflammation 2016 Jan 5;13:2
Journal of neuroinflammation 2016 Jan 5;13:2
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Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Lane 1: mouse embryo lysates Lane 2: mouse brain lysates probed with Anti Phospho-Smad3(Ser423/425) Polyclonal Antibody, Unconjugated (bs-3425R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band 47kD. Observed band size: 47kD.
- Sample type
- Mouse
- Other comments
- Brain
Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded mouse uterus labeled with Anti-Smad3 (Ser423 + Ser425) Polyclonal Antibody, Unconjugated (bs-3425R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
- Sample type
- Mouse
- Other comments
- Uterus