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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Western blot [1]
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- Product number
- bs-5459R - Provider product page
- Provider
- Bioss
- Proper citation
- Bioss Cat#bs-5459R, RRID:AB_11043256
- Product name
- Smad3 (Ser213) Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser213 [PM(p-S)PA]
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1ug/ul
- Storage
- Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Submitted references ATP synthase subunit-β down-regulation aggravates diabetic nephropathy.
Epigallocatechin-3-gallate attenuates unilateral ureteral obstruction-induced renal interstitial fibrosis in mice.
Alantolactone induces apoptosis in HepG2 cells through GSH depletion, inhibition of STAT3 activation, and mitochondrial dysfunction.
Guan SS, Sheu ML, Wu CT, Chiang CK, Liu SH
Scientific reports 2015 Oct 9;5:14561
Scientific reports 2015 Oct 9;5:14561
Epigallocatechin-3-gallate attenuates unilateral ureteral obstruction-induced renal interstitial fibrosis in mice.
Wang Y, Wang B, Du F, Su X, Sun G, Zhou G, Bian X, Liu N
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2015 Apr;63(4):270-9
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2015 Apr;63(4):270-9
Alantolactone induces apoptosis in HepG2 cells through GSH depletion, inhibition of STAT3 activation, and mitochondrial dysfunction.
Khan M, Li T, Ahmad Khan MK, Rasul A, Nawaz F, Sun M, Zheng Y, Ma T
BioMed research international 2013;2013:719858
BioMed research international 2013;2013:719858
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Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Lane 1: mouse brain lysates Lane 2: mouse embryo lysates probed with Anti phospho-Smad3 (Ser213) Polyclonal Antibody, Unconjugated (bs-5459R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band 47kD. Observed band size: 47kD.
- Sample type
- Mouse
- Other comments
- Brain
