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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX111123 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111123, RRID:AB_2038001
- Product name
- SMAD2 / SMAD3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Antroquinonol Targets FAK-Signaling Pathway Suppressed Cell Migration, Invasion, and Tumor Growth of C6 Glioma.
Thiyagarajan V, Tsai MJ, Weng CF
PloS one 2015;10(10):e0141285
PloS one 2015;10(10):e0141285
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Jurkat 7.5% SDS PAGE GTX111123 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SMAD2 / SMAD3 antibody detects SMAD2 / SMAD3 protein by western blot analysis. Mouse tissue extracts (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMAD2 / SMAD3 (GTX111123) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SMAD3 antibody immunoprecipitates SMAD3 protein in IP experiments. IP Sample: 1000 ?g HeLa whole cell lysate/extract A. 50 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of SMAD3protein by 2 £gg of SMAD3antibody (GTX111123) 10% SDS-PAGE The immunoprecipitated SMAD3protein was detected by SMAD3antibody (GTX111123) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of SMAD2 / SMAD3 protein from HeLa whole cell extracts using 2 ?g of SMAD2 / SMAD3 antibody (GTX111123).Western blot analysis was performed using SMAD2 / SMAD3 antibody (GTX111123).EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SMAD2 / SMAD3 antibody detects SMAD2 / SMAD3 protein at cytosol and nucleus on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. SMAD2 / SMAD3 antibody (GTX111123) dilution: 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SMAD2 / SMAD3 antibody immunoprecipitates SMAD2 / SMAD3 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 £gg preimmune rabbit IgG B. 5 £gg of SMAD2 / SMAD3 antibody (GTX111123) The precipitated DNA was detected by PCR with primer set targeting to smad7 promoter.
