Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- OAAB06596 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- SMAD3 Antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
Submitted references SMAD3 functions as a transcriptional repressor of acid-sensing ion channel 3 (ASIC3) in nucleus pulposus cells of the intervertebral disc.
Uchiyama Y, Guttapalli A, Gajghate S, Mochida J, Shapiro IM, Risbud MV
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2008 Oct;23(10):1619-28
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2008 Oct;23(10):1619-28
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of hSMAD3-S208 in MDA-MB231 cell lysates (35ug/lane). SMAD3 (arrow) was detected using the purified Pab.
- Sample type
- MDA-MB231 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- SMAD3-S208 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMAD3-S208 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Sample type
- brain tissue
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol