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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- ARG51797 - Provider product page
- Provider
- Arigo
- Product name
- anti-Smad3 phospho (Ser425) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide sequence around phosphorylation site of serine 425 (C-S-S-V-S(p)) derived from Human Smad3.
- Description
- Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from HeLa cells untreated or treated with EGF stained with anti-SMAD3 (phospho Ser425) antibody ARG51797.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-SMAD3 (phospho Ser425) antibody ARG51797 (left) or the same antibody preincubated with blocking peptide (right).
