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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00004088-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004088-M02, RRID:AB_548205
- Product name
- Mouse Monoclonal Smad3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SMAD3 - SMAD, mothers against DPP homolog 3 (Drosophila)
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Ectopic expression of homeobox gene NKX2-1 in diffuse large B-cell lymphoma is mediated by aberrant chromatin modifications.
Nagel S, Ehrentraut S, Tomasch J, Quentmeier H, Meyer C, Kaufmann M, Drexler HG, MacLeod RA
PloS one 2013;8(4):e61447
PloS one 2013;8(4):e61447
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - Analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M02), clone 7F3.Lane 1: SMAD3 transfected lysate(48 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in PC-12.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in HeLa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in 293.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Smad3 Antibody (7F3) [H00004088-M02] - Detection limit for recombinant GST tagged SMAD3 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Smad3 Antibody (7F3) [H00004088-M02] - Analysis of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
