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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-77836 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77836, RRID:AB_11031542
- Product name
- Rabbit Polyclonal Smad3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Porcine, Xenopus, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Correction of arginine metabolism with sepiapterin-the precursor of nitric oxide synthase cofactor BH(4)-induces immunostimulatory-shift of breast cancer.
Differences in the timing and magnitude of Pkd1 gene deletion determine the severity of polycystic kidney disease in an orthologous mouse model of ADPKD.
TRAF6 mediates Smad-independent activation of JNK and p38 by TGF-beta.
Zheng X, Fernando V, Sharma V, Walia Y, Letson J, Furuta S
Biochemical pharmacology 2020 Jun;176:113887
Biochemical pharmacology 2020 Jun;176:113887
Differences in the timing and magnitude of Pkd1 gene deletion determine the severity of polycystic kidney disease in an orthologous mouse model of ADPKD.
Rogers KA, Moreno SE, Smith LA, Husson H, Bukanov NO, Ledbetter SR, Budman Y, Lu Y, Wang B, Ibraghimov-Beskrovnaya O, Natoli TA
Physiological reports 2016 Jun;4(12)
Physiological reports 2016 Jun;4(12)
TRAF6 mediates Smad-independent activation of JNK and p38 by TGF-beta.
Yamashita M, Fatyol K, Jin C, Wang X, Liu Z, Zhang YE
Molecular cell 2008 Sep 26;31(6):918-24
Molecular cell 2008 Sep 26;31(6):918-24
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF (+lanes) for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein. Personal Communication. YIgG Zhang, NIH, CCR, Bethesda, MD.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong nuclear staining in the majority of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
