Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- Ab128212 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant SMAD3 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human SMAD3 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.Application:WB: 1/500-1/1000IHC: 1/50-1/100ICC/IF: 1/50-1/200Protein FunctionReceptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 202-219
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant SMAD3 Antibody (Ab128212) - Western Blot All lanes: Recombinant SMAD3 Antibody (Ab128212) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 51 kDa Exposure time: 112.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant SMAD3 Antibody (Ab128212) - IF Immunocytochemistry analysis of Smad3 (green) in A549 cells using Recombinant SMAD3 Antibody (Ab128212) and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant SMAD3 Antibody (Ab128212) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant SMAD3 Antibody (Ab128212). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.