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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP53938PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53938PU-N, RRID:AB_11148347
- Product name
- anti SMAD3
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide selected from the Human SMAD3.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of SMAD3 Antibody Cat.-No AP53938PU-N in A549 cell line lysates (35ug/lane). This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin fixed, paraffin embedded human thyroid tissue stained with SMAD3 Antibody Cat.-No AP53938PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SMAD3 Antibody (S213) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A549 cells using SMAD3 Antibody Cat.-No AP53938PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
