Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP53939PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53939PU-N, RRID:AB_11148971
- Product name
- anti SMAD3
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide selected from the Ser208 region of Human SMAD3
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of SMAD3 Antibody Cat.-No AP53939PU-NÂ in MDA-MB231 cell line lysates (35ug/lane). SMAD3 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of SMAD3 Antibody Cat.-No AP53939PU-N with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- formalin fixed, paraffin embedded brain tissue stained with SMAD3 Antibody Cat.-No AP53939PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMAD3-S208 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MDA-MB231 cells using SMAD3 Antibody Cat.-No AP53939PU-N (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
