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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN954841 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (Ser208) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide selected from the Ser208 region of Human SMAD3
- Description
- Affinity Chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- Ser208
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Smad3 prevents beta-catenin degradation and facilitates beta-catenin nuclear translocation in chondrocytes.
Hypoxia-activated Smad3-specific dephosphorylation by PP2A.
Smad3 protein levels are modulated by Ras activity and during the cell cycle to dictate transforming growth factor-beta responses.
Association of TGFBR2 polymorphism with risk of sudden cardiac arrest in patients with coronary artery disease.
Zhang M, Wang M, Tan X, Li TF, Zhang YE, Chen D
The Journal of biological chemistry 2010 Mar 19;285(12):8703-10
The Journal of biological chemistry 2010 Mar 19;285(12):8703-10
Hypoxia-activated Smad3-specific dephosphorylation by PP2A.
Heikkinen PT, Nummela M, Leivonen SK, Westermarck J, Hill CS, Kähäri VM, Jaakkola PM
The Journal of biological chemistry 2010 Feb 5;285(6):3740-9
The Journal of biological chemistry 2010 Feb 5;285(6):3740-9
Smad3 protein levels are modulated by Ras activity and during the cell cycle to dictate transforming growth factor-beta responses.
Daly AC, Vizán P, Hill CS
The Journal of biological chemistry 2010 Feb 26;285(9):6489-97
The Journal of biological chemistry 2010 Feb 26;285(9):6489-97
Association of TGFBR2 polymorphism with risk of sudden cardiac arrest in patients with coronary artery disease.
Tseng ZH, Vittinghoff E, Musone SL, Lin F, Whiteman D, Pawlikowska L, Kwok PY, Olgin JE, Aouizerat BE
Heart rhythm : the official journal of the Heart Rhythm Society 2009 Dec;6(12):1745-50
Heart rhythm : the official journal of the Heart Rhythm Society 2009 Dec;6(12):1745-50
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of SMAD3 Antibody Cat.-No AP53939PU-N in MDA-MB231 cell line lysates (35ug/lane). SMAD3 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of SMAD3 Antibody Cat.-No AP53939PU-N with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- formalin fixed, paraffin embedded brain tissue stained with SMAD3 Antibody Cat.-No AP53939PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMAD3-S208 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MDA-MB231 cells using SMAD3 Antibody Cat.-No AP53939PU-N (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
