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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-48073 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FSTL4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody detects recombinant mouse Follistatin-like 4/FSTL4 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) FSTL4 is observed, and less than 5% cross-reactivity with rhFSTL5 and recombinant mouse FSTL1 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of mouse brain (cortex) tissue, rat brain (cortex) tissue, and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-mouse Follistatin-like 4/FSTL4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-48073) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Follistatin-like 4/FSTL4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FSTL4 in mouse brain (cortex) tissue, rat brain (cortex) tissue, and human brain (hippocampus) tissue. Samples were incubated in FSTL4 polyclonal antibody (Product # PA5-48073) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Follistatin-like 4/FSTL4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FSTL4 in perfusion fixed frozen sections of mouse brain. Samples were incubated in FSTL4 polyclonal antibody (Product # PA5-48073) using a dilution of 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons in the brainstem. This application has not been tested in human or rat samples.
