Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- 19097-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LINGO1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: SGSATGCPP RCECSAQDRA VLCHRKRFVA VPEGIPTETR LLDLGKNRIK TLNQDEFASF PHLEELELNE NIVSAVEPGA FNNLFNLRTL GLRSNRLKLI PLGVFTGLSN LTKLDISENK IVILLDYMFQ DLYNLKSLEV GDNDLVYISH RAFSGLNSLE QLTLEKCNLT SIPTEALSHL HGLIVLRLRH LNINAIRDYS FKRLYRLKVL EISHWPYLDT MTPNCLYGLN LTSLSITHCN LTAVPYLAVR HLVYLRFLNL SYNPISTIEG SMLHELLRLQ EIQLVGGQLA VVEPYAFRGL NYLRVLNVSG NQLTTLE (36-351 aa encoded by BC011057)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 19097-1-AP (LINGO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 19097-1-AP (LINGO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 SH-SY5Y cells were stained with 0.2ug LINGO1 antibody (19097-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.