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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- PA5-60294 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GCDH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SRPEFDWQDP LVLEEQLTTD EILIRDTFRT YCQERLMPRI LLANRNEVFH REIISEMGEL GVLGPTIKGY GCAGV
- Concentration
- 0.5 mg/mL
Submitted references Deglutarylation of glutaryl-CoA dehydrogenase by deacylating enzyme SIRT5 promotes lysine oxidation in mice.
Bhatt DP, Mills CA, Anderson KA, Henriques BJ, Lucas TG, Francisco S, Liu J, Ilkayeva OR, Adams AE, Kulkarni SR, Backos DS, Major MB, Grimsrud PA, Gomes CM, Hirschey MD
The Journal of biological chemistry 2022 Apr;298(4):101723
The Journal of biological chemistry 2022 Apr;298(4):101723
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GCDH using GCDH Polyclonal Antibody (Product # PA5-60294) (A) shows similar pattern to an independent GCDH Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of GCDH in human cell line U-2 OS shows positivity in mitochondria. Samples were probed using a GCDH Polyclonal Antibody (Product # PA5-60294).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GCDH in human liver using a GCDH Polyclonal Antibody (Product # PA5-60294) shows strong cytoplasmic positivity in hepatocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GCDH in human kidney, liver, pancreas and testis using GCDH Polyclonal Antibody (Product # PA5-60294) (A) shows similar protein distribution across tissues to an independent GCDH Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GCDH in human pancreas using GCDH Polyclonal Antibody (Product # PA5-60294) shows moderate granular cytoplasmic positivity in exocrine glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GCDH in human kidney using GCDH Polyclonal Antibody (Product # PA5-60294) shows strong granular cytoplasmic positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GCDH in human testis using GCDH Polyclonal Antibody (Product # PA5-60294) shows weak granular cytoplasmic positivity.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GCDH in human liver using GCDH Polyclonal Antibody (Product # PA5-60294) shows strong granular cytoplasmic positivity in hepatocytes.
