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LearnA301-274A
antibody from Invitrogen Antibodies
Targeting: INTS11
CPSF3L, CPSF73L, FLJ20542, INT11, RC-68
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A301-274A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- INT11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse INT11 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, K-562, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT11 antibody (Product # A301-274A; lot A301-274A-4) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human INT11 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INT11 antibody A301-274A (lot A301-274A-3) used for IP at 6 µg per reaction. INT11 was also immunoprecipitated by a previous lot of this antibody (lot A301-274A-2) and rabbit anti-INT11 antibody BL5185. For blotting immunoprecipitated INT11, A301-274A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse INT11 by western blot. Samples: Whole cell lysate from HeLa (15 µg), 293T (50 µg), mouse TCMK-1 (50 µg), and mouse NIH3T3 (50 µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT11 antibody A301-274A (lot A301-274A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human INT11 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-INT11 (Product # A301-274A; lot 4) used at a dilution of 1:5,000 (0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human INT11 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-INT11 (Cat. No. A301-274A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human INT11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INT11 antibody (Product # A301-274A; lot A301-274A-4) used for IP at 6 µg per reaction. INT11 was also immunoprecipitated by a previous lot of this antibody (lot A301-274A-3). For blotting immunoprecipitated INT11 (Product # A301-274A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
