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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX105167 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105167, RRID:AB_11168499
- Product name
- HADH antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HADH antibody detects HADH protein by Western blot analysis.A. 30 ?g GL261 whole cell lysate/extractB. 30 ug C8D30 whole cell lysate/extract10 % SDS-PAGEHADH antibody (GTX105167) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HADH antibody detects HADH protein by Western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract10 % SDS-PAGEHADH antibody (GTX105167) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HADH antibody detects HADH protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extractD. 30 ug HepG2 whole cell lysate/extract10 % SDS-PAGEHADH antibody (GTX105167) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HADH antibody detects HADH protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: HADH protein stained by HADH antibody (GTX105167) diluted at 1:500.Blue: Hoechst 33343 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HADH antibody detects HADH protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: HADH protein stained by HADH antibody (GTX105167) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of HADH protein from HepG2 whole cell extracts using 5 £gg of HADH antibody (GTX105167).Western blot analysis was performed using HADH antibody (GTX105167).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HADH antibody detects HADH protein at mitochondria on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HADH antibody (GTX105167) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HADH antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. HADH antibody (GTX105167) dilution: 1:500.
