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- Antibody Data
- Antigen structure
- References [3]
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- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-28203 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HADH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, GL261, C8D30, PC-12.
- Concentration
- 1 mg/mL
Submitted references Short-Term High-Fat Feeding Does Not Alter Mitochondrial Lipid Respiratory Capacity but Triggers Mitophagy Response in Skeletal Muscle of Mice.
Robust intrinsic differences in mitochondrial respiration and H(2)O(2) emission between L6 and C2C12 cells.
Long-term rates of mitochondrial protein synthesis are increased in mouse skeletal muscle with high-fat feeding regardless of insulin-sensitizing treatment.
Ehrlicher SE, Stierwalt HD, Newsom SA, Robinson MM
Frontiers in endocrinology 2021;12:651211
Frontiers in endocrinology 2021;12:651211
Robust intrinsic differences in mitochondrial respiration and H(2)O(2) emission between L6 and C2C12 cells.
Robinson MM, Sather BK, Burney ER, Ehrlicher SE, Stierwalt HD, Franco MC, Newsom SA
American journal of physiology. Cell physiology 2019 Aug 1;317(2):C339-C347
American journal of physiology. Cell physiology 2019 Aug 1;317(2):C339-C347
Long-term rates of mitochondrial protein synthesis are increased in mouse skeletal muscle with high-fat feeding regardless of insulin-sensitizing treatment.
Newsom SA, Miller BF, Hamilton KL, Ehrlicher SE, Stierwalt HD, Robinson MM
American journal of physiology. Endocrinology and metabolism 2017 Nov 1;313(5):E552-E562
American journal of physiology. Endocrinology and metabolism 2017 Nov 1;313(5):E552-E562
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HADH Polyclonal Antibody detects HADH protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract.10 % SDS-PAGE. HADH Polyclonal Antibody (Product # PA5-28203) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HADH Polyclonal Antibody detects HADH protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract. B. 30 µg C8D30 whole cell lysate/extract.10 % SDS-PAGE. HADH Polyclonal Antibody (Product # PA5-28203) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HADH Polyclonal Antibody detects HADH protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.10 % SDS-PAGE. HADH Polyclonal Antibody (Product # PA5-28203) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HADH was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: HADH Polyclonal Antibody (Product # PA5-28203) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HADH Polyclonal Antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. HADH Polyclonal Antibody (Product # PA5-28203) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HADH Polyclonal Antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. HADH Polyclonal Antibody (Product # PA5-28203) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HADH was performed in HepG2 whole cell extracts using 5 µg of HADH Polyclonal Antibody (Product # PA5-28203). Samples were transferred to a membrane and probed with HADH Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Short-term high-fat feeding did not alter mitochondrial enzyme abundance. Immunoblotting of lysates from quadricep muscle collected after a 4-hour fast from mice fed low-fat diet or high-fat diet for 3 days, 7 days, or 12 weeks. (A) Protein abundance of subunits in mitochondrial respiratory complexes I-V expressed in arbitrary units. (B) Hadh protein abundance in tissue lysates. (C) Representative blot images for (A, B) . Data are means +- SD; n=4-5. Two-way ANOVA was used to test for effects of diet and time. P values are main effects.
