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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AM00095PU-N - Provider product page
- Provider
- OriGene
- Product name
- MEK5 (MAP2K5) (incl. pos. control) mouse monoclonal antibody, clone 14B5, Purified
- Antibody type
- Monoclonal
- Description
- MEK5 (MAP2K5) (incl. pos. control) mouse monoclonal antibody, clone 14B5, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MAP2K5
- Isotype
- IgG
- Antibody clone number
- 14B5
- Vial size
- 100 µg
- Concentration
- NULL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Detection of endogenous MKK5 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MKK5-14B5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
- Validation comment
- WB
