Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00005607-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005607-M01, RRID:AB_425621
- Product name
- MAP2K5 monoclonal antibody (M01), clone M1-E6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant MAP2K5.
- Antigen sequence
MLWLALGPFPAMENQVLVIRIKIPNSGAVDWTVHS
GPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRI
TVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPLQIF
PRACKPPGERNIHGLKVNTRAGPSQHSSPAVSDSL
PSNSLKKSSAELKKILANGQMNEQDIRYRDTLGHG
NGGTVYKAYHVPSGKILAVKVILLDITLELQKQIM
SELEILYKCDSSYIIGFYGAFFVENRISICTEFMD
GGSLDVYRKMPEHVLGRIAVAVVKGLTYLWSLKIL
HRDVKPSNMLVNTRGQVKLCDFGVSTQLVNSIAKT
YVGTNAYMAPERISGEQYGIHSDVWSLGISFMELA
LGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPV
GESSEPFVHFITQCMRKQPKERPAPEELMGHPFIV
QFNDGNAAVVSMWVCRALEERRSQQGPP- Isotype
- IgG
- Antibody clone number
- M1-E6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MAP2K5 monoclonal antibody (M01), clone M1-E6 Western Blot analysis of MAP2K5 expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MAP2K5 monoclonal antibody (M01), clone M1-E6. Western Blot analysis of MAP2K5 expression in K-562 ( Cat # L009V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of MAP2K5 expression in transfected 293T cell line by MAP2K5 monoclonal antibody (M01), clone M1-E6.Lane 1: MAP2K5 transfected lysate (Predicted MW: 50.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged MAP2K5 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to MAP2K5 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol