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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2270 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2270, RRID:AB_1577153
- Product name
- MAP2K5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MAP2K5.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references MEK5 and ERK5 are localized in the nuclei of resting as well as stimulated cells, while MEKK2 translocates from the cytosol to the nucleus upon stimulation.
Contribution of the ERK5/MEK5 pathway to Ras/Raf signaling and growth control.
BMK1/ERK5 regulates serum-induced early gene expression through transcription factor MEF2C.
Raviv Z, Kalie E, Seger R
Journal of cell science 2004 Apr 1;117(Pt 9):1773-84
Journal of cell science 2004 Apr 1;117(Pt 9):1773-84
Contribution of the ERK5/MEK5 pathway to Ras/Raf signaling and growth control.
English JM, Pearson G, Hockenberry T, Shivakumar L, White MA, Cobb MH
The Journal of biological chemistry 1999 Oct 29;274(44):31588-92
The Journal of biological chemistry 1999 Oct 29;274(44):31588-92
BMK1/ERK5 regulates serum-induced early gene expression through transcription factor MEF2C.
Kato Y, Kravchenko VV, Tapping RI, Han J, Ulevitch RJ, Lee JD
The EMBO journal 1997 Dec 1;16(23):7054-66
The EMBO journal 1997 Dec 1;16(23):7054-66
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MAP2K5 (arrow) using rabbit MAP2K5 polyclonal antibody (Cat # PAB2270). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K5 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K5 polyclonal antibody (Cat # PAB2270) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
