PA5-15319

antibody from Invitrogen Antibodies

Targeting: DGKZ DAGK5, DAGK6, DGK-ZETA, hDGKzeta

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-15319

Provider

Invitrogen Antibodies

Product name

DGKZ Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.45 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DGKZ (arrow) using a DGKZ polyclonal antibody (Product # PA5-15319) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the DGKZ gene (Lane 2).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a DGKZ polyclonal antibody (Product # PA5-15319) in Ramos cell lysates (35 µg per lane).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DGKZ (arrow) using a DGKZ polyclonal antibody (Product # PA5-15319) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the DGKZ gene (Lane 2).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis using a DGKZ polyclonal antibody (Product # PA5-15319). Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with a SF1 antibody and a DGKZ polyclonal antibody (Product # PA5-15319) (B) for 1 h. Immunofluorescence was detected by confocal microscopy.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a DGKZ polyclonal antibody (Product # PA5-15319), followed by HRP-conjugated secondary antibody and AEC staining.