Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-22360 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TSSC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116. Predicted reactivity: Mouse (90%), Rat (90%), Zebrafish (83%), Xenopus laevis (82%), Pig (83%), Chicken (92%), Bovine (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.75 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references EIPR1 controls dense-core vesicle cargo retention and EARP complex localization in insulin-secreting cells.
Topalidou I, Cattin-Ortolá J, Hummer B, Asensio CS, Ailion M
Molecular biology of the cell 2020 Jan 1;31(1):59-79
Molecular biology of the cell 2020 Jan 1;31(1):59-79
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TSSC1 Polyclonal Antibody (Product # PA5-22360). Sample (30 µg of whole cell lysate). Lane A: Hela. 10% SDS PAGE. TSSC1 Polyclonal Antibody (Product # PA5-22360) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TSSC1 in methanol-fixed HeLa cells using a TSSC1 polyclonal antibody (Product # PA5-22360) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 1: Insulin secretion is reduced in Eipr1 KO cells. (A) Strategy used to create the Eipr1 KO 832/13 cell line. Cas9 was targeted to cut in the first exon of the rat Eipr1 locus and homologous recombination (HR) was used to insert a puromycin cassette. (B) Eipr1 KO cells do not express wild-type (WT) EIPR1. Protein extracts from 832/13 (WT), Eipr1 KO 832/13 (Eipr1 KO), and Eipr1 KO 832/13 cells expressing a WT Eipr1 cDNA (EIPR1[+]) were blotted with an EIPR1 antibody. beta-Tubulin served as a loading control. (C) Left panel, insulin secretion under resting (5 mM KCl, 0 mM glucose) and stimulating conditions (55 mM KCl, 25 mM glucose) from 832/13 cells (WT), Eipr1 KO 832/13 cells (Eipr1 KO), and an Eipr1 KO stable line expressing WT Eipr1 (EIPR1[+]). All values were normalized to the value of the WT under stimulating conditions. n = 7; *, p < 0.05; **, p < 0.01; ns, p > 0.05; error bars = SEM. Middle panel, total insulin content in WT, Eipr1 KO, and EIPR1(+) cells. All values were normalized to the WT. n = 5-7; ns, p > 0.05; error bars = SEM. Right panel, insulin secretion normalized to insulin content under resting (5 mM KCl, 0 mM glucose) and stimulating conditions (55 mM KCl, 25 mM glucose) from WT, Eipr1 KO, and EIPR1(+) cells. n = 5-7; *, p < 0.05; **, p < 0.01; ns, p > 0.05; error bars = SEM. We performed three biological replicates. For each replicate, the same cells were used to determine the amount of insulin secreted u