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antibody from Novus Biologicals
Targeting: HLA-E
ELISA Immunocytochemistry Immunoprecipitation
Immunohistochemistry Flow cytometry Blocking/Neutralizing Immunoelectron microscopyAntibody data
- Antibody Data
- Antigen structure
- References [29]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- NB120-6405 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-6405, RRID:AB_791151
- Product name
- Mouse Monoclonal MHC Class I Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognised.
- Reactivity
- Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Transcranial Current Stimulation Alters the Expression of Immune-Mediating Genes.
Interleukin-17 impedes Schwann cell-mediated myelination.
Influence of perineurial cells and Toll-like receptors 2 and 9 on Herpes simplex type 1 entry to the central nervous system in rat encephalitis.
Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells.
A role for the thiol-dependent reductase ERp57 in the assembly of MHC class I molecules.
Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro.
Bone marrow derived elements and resident microglia in brain inflammation.
Membrane-bound and water-soluble nonclassical class I MHC antigens on rat placenta.
Intrathecal application of interferon gamma. Progressive appearance of MHC antigens within the rat nervous system.
Antigen-specific damage to brain vascular endothelial cells mediated by encephalitogenic and nonencephalitogenic CD4+ T cell lines in vitro.
Concerted evolution of class I genes in the major histocompatibility complex of murine rodents.
Early major histocompatibility complex (MHC) class I antigen induction in hypothalamic supraoptic and paraventricular nuclei in trypanosome-infected rats.
Class II antigens on retinal vascular endothelium, pericytes, macrophages, and lymphocytes of the rat.
Macrophage infiltration precedes and is a prerequisite for lymphocytic insulitis in pancreatic islets of pre-diabetic BB rats.
Oligodendrocyte-specific autoreactive T cells using an alpha/beta T-cell receptor kill their target without self restriction.
Mercuric chloride-induced autoimmunity in the brown Norway rat. Cellular kinetics and major histocompatibility complex antigen expression.
Effect of Cyclosporin A on rat thymus: time course analysis by immunoperoxidase technique and flow cytofluorometry.
The relationship of modulation of major histocompatibility complex class I antigens to retrovirus transformation in rat cell lines.
The effect of interferon-gamma treatment of rat tumour cells on their susceptibility to natural killer cell, macrophage and cytotoxic T-cell killing.
The differentiation of O-2A progenitor cells into oligodendrocytes is associated with a loss of inducibility of Ia antigens.
Identification in rat liver and serum of water-soluble class I MHC molecules possibly homologous to the murine Q10 gene product.
Regulation of major histocompatibility complex class II antigen expression by the FRTL-5 rat thyroid cell line.
In situ detection of class I and II major histocompatibility complex antigens in the rat central nervous system during experimental allergic encephalomyelitis. An immunohistochemical study.
Inhibition of IL 2-dependent proliferation of rat T lymphoblasts by the monoclonal antibody ART62 which reacts with MHC class 1 antigens.
Development of graft-vs.-host disease-like syndrome in cyclosporine-treated rats after syngeneic bone marrow transplantation. I. Development of cytotoxic T lymphocytes with apparent polyclonal anti-Ia specificity, including autoreactivity.
Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy.
Ia antigens in rat kidney, with special reference to their expression in tubular epithelium.
The distribution, ontogeny and origin in the rat of Ia-positive cells with dendritic morphology and of Ia antigen in epithelia, with special reference to the intestine.
Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus.
Rabenstein M, Unverricht-Yeboah M, Keuters MH, Pikhovych A, Hucklenbroich J, Vay SU, Blaschke S, Ladwig A, Walter HL, Beiderbeck M, Fink GR, Schroeter M, Kriehuber R, Rueger MA
Frontiers in cellular neuroscience 2019;13:461
Frontiers in cellular neuroscience 2019;13:461
Interleukin-17 impedes Schwann cell-mediated myelination.
Stettner M, Lohmann B, Wolffram K, Weinberger JP, Dehmel T, Hartung HP, Mausberg AK, Kieseier BC
Journal of neuroinflammation 2014 Mar 29;11:63
Journal of neuroinflammation 2014 Mar 29;11:63
Influence of perineurial cells and Toll-like receptors 2 and 9 on Herpes simplex type 1 entry to the central nervous system in rat encephalitis.
Bereczky-Veress B, Abdelmagid N, Piehl F, Bergström T, Olsson T, Sköldenberg B, Diez M
PloS one 2010 Aug 27;5(8):e12350
PloS one 2010 Aug 27;5(8):e12350
Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells.
Baca Jones CC, Kreklywich CN, Messaoudi I, Vomaske J, McCartney E, Orloff SL, Nelson JA, Streblow DN
Virology 2009 May 25;388(1):78-90
Virology 2009 May 25;388(1):78-90
A role for the thiol-dependent reductase ERp57 in the assembly of MHC class I molecules.
Morrice NA, Powis SJ
Current biology : CB 1998 Jun 4;8(12):713-6
Current biology : CB 1998 Jun 4;8(12):713-6
Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro.
Zhai Y, Knechtle S
Human immunology 1998 Jul;59(7):404-14
Human immunology 1998 Jul;59(7):404-14
Bone marrow derived elements and resident microglia in brain inflammation.
Lassmann H, Schmied M, Vass K, Hickey WF
Glia 1993 Jan;7(1):19-24
Glia 1993 Jan;7(1):19-24
Membrane-bound and water-soluble nonclassical class I MHC antigens on rat placenta.
Fowler VJ, Dalchau SR, Fabre JW
Immunogenetics 1990;31(3):152-60
Immunogenetics 1990;31(3):152-60
Intrathecal application of interferon gamma. Progressive appearance of MHC antigens within the rat nervous system.
Vass K, Lassmann H
The American journal of pathology 1990 Oct;137(4):789-800
The American journal of pathology 1990 Oct;137(4):789-800
Antigen-specific damage to brain vascular endothelial cells mediated by encephalitogenic and nonencephalitogenic CD4+ T cell lines in vitro.
Sedgwick JD, Hughes CC, Male DK, MacPhee IA, ter Meulen V
Journal of immunology (Baltimore, Md. : 1950) 1990 Oct 15;145(8):2474-81
Journal of immunology (Baltimore, Md. : 1950) 1990 Oct 15;145(8):2474-81
Concerted evolution of class I genes in the major histocompatibility complex of murine rodents.
Rada C, Lorenzi R, Powis SJ, van den Bogaerde J, Parham P, Howard JC
Proceedings of the National Academy of Sciences of the United States of America 1990 Mar;87(6):2167-71
Proceedings of the National Academy of Sciences of the United States of America 1990 Mar;87(6):2167-71
Early major histocompatibility complex (MHC) class I antigen induction in hypothalamic supraoptic and paraventricular nuclei in trypanosome-infected rats.
Schultzberg M, Olsson T, Samuelsson EB, Maehlen J, Kristensson K
Journal of neuroimmunology 1989 Sep;24(1-2):105-12
Journal of neuroimmunology 1989 Sep;24(1-2):105-12
Class II antigens on retinal vascular endothelium, pericytes, macrophages, and lymphocytes of the rat.
Fujikawa LS, Reay C, Morin ME
Investigative ophthalmology & visual science 1989 Jan;30(1):66-73
Investigative ophthalmology & visual science 1989 Jan;30(1):66-73
Macrophage infiltration precedes and is a prerequisite for lymphocytic insulitis in pancreatic islets of pre-diabetic BB rats.
Hanenberg H, Kolb-Bachofen V, Kantwerk-Funke G, Kolb H
Diabetologia 1989 Feb;32(2):126-34
Diabetologia 1989 Feb;32(2):126-34
Oligodendrocyte-specific autoreactive T cells using an alpha/beta T-cell receptor kill their target without self restriction.
Jewtoukoff V, Lebar R, Bach MA
Proceedings of the National Academy of Sciences of the United States of America 1989 Apr;86(8):2824-8
Proceedings of the National Academy of Sciences of the United States of America 1989 Apr;86(8):2824-8
Mercuric chloride-induced autoimmunity in the brown Norway rat. Cellular kinetics and major histocompatibility complex antigen expression.
Aten J, Bosman CB, Rozing J, Stijnen T, Hoedemaeker PJ, Weening JJ
The American journal of pathology 1988 Oct;133(1):127-38
The American journal of pathology 1988 Oct;133(1):127-38
Effect of Cyclosporin A on rat thymus: time course analysis by immunoperoxidase technique and flow cytofluorometry.
Tanaka M, Shinohara K, Fukumoto T, Tanaka H, Kaneko T
Clinical and experimental immunology 1988 May;72(2):216-21
Clinical and experimental immunology 1988 May;72(2):216-21
The relationship of modulation of major histocompatibility complex class I antigens to retrovirus transformation in rat cell lines.
Racioppi L, Carbone E, Grieco M, Del Vecchio L, Berlingieri MT, Fusco A, Boncinelli E, Zappacosta S, Fontana S
Cancer research 1988 Jul 1;48(13):3816-21
Cancer research 1988 Jul 1;48(13):3816-21
The effect of interferon-gamma treatment of rat tumour cells on their susceptibility to natural killer cell, macrophage and cytotoxic T-cell killing.
Yeoman H, Robins RA
Immunology 1988 Feb;63(2):291-7
Immunology 1988 Feb;63(2):291-7
The differentiation of O-2A progenitor cells into oligodendrocytes is associated with a loss of inducibility of Ia antigens.
Calder VL, Wolswijk G, Noble M
European journal of immunology 1988 Aug;18(8):1195-201
European journal of immunology 1988 Aug;18(8):1195-201
Identification in rat liver and serum of water-soluble class I MHC molecules possibly homologous to the murine Q10 gene product.
Spencer SC, Fabre JW
The Journal of experimental medicine 1987 Jun 1;165(6):1595-608
The Journal of experimental medicine 1987 Jun 1;165(6):1595-608
Regulation of major histocompatibility complex class II antigen expression by the FRTL-5 rat thyroid cell line.
Weetman AP, Green C, Borysiewicz LK
The Journal of endocrinology 1987 Dec;115(3):481-7
The Journal of endocrinology 1987 Dec;115(3):481-7
In situ detection of class I and II major histocompatibility complex antigens in the rat central nervous system during experimental allergic encephalomyelitis. An immunohistochemical study.
Matsumoto Y, Fujiwara M
Journal of neuroimmunology 1986 Oct;12(4):265-77
Journal of neuroimmunology 1986 Oct;12(4):265-77
Inhibition of IL 2-dependent proliferation of rat T lymphoblasts by the monoclonal antibody ART62 which reacts with MHC class 1 antigens.
Osawa H, Herrmann T, Diamantstein T
Journal of immunology (Baltimore, Md. : 1950) 1985 Jun;134(6):3901-6
Journal of immunology (Baltimore, Md. : 1950) 1985 Jun;134(6):3901-6
Development of graft-vs.-host disease-like syndrome in cyclosporine-treated rats after syngeneic bone marrow transplantation. I. Development of cytotoxic T lymphocytes with apparent polyclonal anti-Ia specificity, including autoreactivity.
Hess AD, Horwitz L, Beschorner WE, Santos GW
The Journal of experimental medicine 1985 Apr 1;161(4):718-30
The Journal of experimental medicine 1985 Apr 1;161(4):718-30
Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy.
Bukovský A, Presl J, Zidovský J
Immunology 1984 Aug;52(4):631-40
Immunology 1984 Aug;52(4):631-40
Ia antigens in rat kidney, with special reference to their expression in tubular epithelium.
Mayrhofer G, Schon-Hegrad MA
The Journal of experimental medicine 1983 Jun 1;157(6):2097-109
The Journal of experimental medicine 1983 Jun 1;157(6):2097-109
The distribution, ontogeny and origin in the rat of Ia-positive cells with dendritic morphology and of Ia antigen in epithelia, with special reference to the intestine.
Mayrhofer G, Pugh CW, Barclay AN
European journal of immunology 1983 Feb;13(2):112-22
European journal of immunology 1983 Feb;13(2):112-22
Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus.
Fukumoto T, McMaster WR, Williams AF
European journal of immunology 1982 Mar;12(3):237-43
European journal of immunology 1982 Mar;12(3):237-43
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Endothelial staining was observed. Staining was performed by Histowiz.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were stained with MHC Class I NB120-6405 (Blue) and a matched isotype control NBP2-27287 (Orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by DyLight488-conjugated anti-mouse secondary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
