H00007320-M06

antibody from Abnova Corporation

Targeting: UBE2B HHR6B, RAD6B, UBC2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007320-M06

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007320-M06, RRID:AB_1137517

Product name

UBE2B monoclonal antibody (M06), clone 4C3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant UBE2B.

Antigen sequence

YPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWS
PTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQE
NKREYEKRVSAIVEQSWNDS


Isotype

IgG

Antibody clone number

4C3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of UBE2B expression in transfected 293T cell line by UBE2B monoclonal antibody (M06), clone 4C3.Lane 1: UBE2B transfected lysate(17.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged UBE2B is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol