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ImmunocytochemistryAntibody data
- Antibody Data
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- References [1]
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- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- NBP1-32725 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32725, RRID:AB_2285570
- Product name
- Rabbit Polyclonal SAR1B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references New Insights In Intestinal Sar1B GTPase Regulation and Role in Cholesterol Homeostasis.
Sané A, Seidman E, Spahis S, Lamantia V, Garofalo C, Montoudis A, Marcil V, Levy E
Journal of cellular biochemistry 2015 Oct;116(10):2270-82
Journal of cellular biochemistry 2015 Oct;116(10):2270-82
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SAR1B Antibody [NBP1-32725] - A. 50 ug rat brain lysate/extract 12 % SDS-PAGE SAR1B antibody dilution: 1:3000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SAR1B Antibody [NBP1-32725] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE, antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SAR1B Antibody [NBP1-32725] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE, antibody diluted at 1:3000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SAR1B Antibody [NBP1-32725] - Adult mouse small intestine homogenate, 20 ug total protein per lane. Antibody at 1:1000. Secondary HRP conjugated antibody at 1:5000. Bands visualized with Immobilon Western Chemiluminescence HRP substrate, imaged with Syngene system. WB image submitted by a verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SAR1B Antibody [NBP1-32725] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SAR1B Antibody [NBP1-32725] - Mouse ovary. SAR1B antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
