600-401-878

antibody from Rockland Immunochemicals, Inc.

Targeting: CCNL2 ania-6b, CCNM, CCNS, HLA-ISO, PCEE, SB138

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-878

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-878, RRID:AB_828131

Product name

Anti-Cyclin L2 (isoform 1) [RABBIT] Antibody - 600-401-878

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

CDK11 Loss Induces Cell Cycle Dysfunction and Death of BRAF and NRAS Melanoma Cells.

Ahmed RL, Shaughnessy DP, Knutson TP, Vogel RI, Ahmed K, Kren BT, Trembley JH
Pharmaceuticals (Basel, Switzerland) 2019 Apr 2;12(2)

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Preclinical evaluation of cyclin dependent kinase 11 and casein kinase 2 survival kinases as RNA interference targets for triple negative breast cancer therapy.

Kren BT, Unger GM, Abedin MJ, Vogel RI, Henzler CM, Ahmed K, Trembley JH
Breast cancer research : BCR 2015;17:19

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's Affinity Purified anti-Cyclin L2a antibody shows detection of a band ~38 kDa corresponding to a GST-truncated human Cyclin L2a fusion protein (arrowhead). Approximately 5 ul of an induced E.coli cell lysate expressing recombinant Cyclin L2 was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000 overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Validation comment

Western Blot