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LearnNB100-87009
antibody from Novus Biologicals
Targeting: CCNL2
ania-6b, CCNM, CCNS, HLA-ISO, PCEE, SB138
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-87009 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-87009, RRID:AB_1201144
- Product name
- Rabbit Polyclonal Cyclin L2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The Dual-Specificity Kinase DYRK1A Modulates the Levels of Cyclin L2 To Control HIV Replication in Macrophages.
Cyclin L2 is a critical HIV dependency factor in macrophages that controls SAMHD1 abundance.
Kisaka JK, Ratner L, Kyei GB
Journal of virology 2020 Feb 28;94(6)
Journal of virology 2020 Feb 28;94(6)
Cyclin L2 is a critical HIV dependency factor in macrophages that controls SAMHD1 abundance.
Kyei GB, Cheng X, Ramani R, Ratner L
Cell host & microbe 2015 Jan 14;17(1):98-106
Cell host & microbe 2015 Jan 14;17(1):98-106
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyclin L2 Antibody [NB100-87009] - Detection of Human Cyclin L2 on HeLa whole cell lysate using NB100-87009. Cyclin L2 was also immunoprecipitated by rabbit anti-Cyclin L2 antibody NB100-87008.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyclin L2 Antibody [NB100-87009] - Whole cell lysate (50 ug) from HEK293T, K-562, HeLa, U2OS, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Cyclin L2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Cyclin L2 Antibody [NB100-87009] - Detection of human Cyclin L2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-Cyclin L2 antibody NB100-87009 (lot 2) used for IP at 6 ug per reaction. Cyclin L2 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-Cyclin L2 antibody from Company B. For blotting immunoprecipitated Cyclin L2, NB100-87009 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
