GTX85686

antibody from GeneTex

Targeting: CCNL2 ania-6b, CCNM, CCNS, HLA-ISO, PCEE, SB138

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX85686

Provider

GeneTex

Proper citation

GeneTex Cat#GTX85686, RRID:AB_10732293

Product name

CCNL2 antibody

Antibody type

Polyclonal

Reactivity

Human, Rat, Chicken/Avian

Host

Rabbit

Storage

+4¢XC or -20¢XC, Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot of Affinity Purified anti-Cyclin L2a antibody shows detection of a band ~38 kDa corresponding to a GST-truncated human Cyclin L2a fusion protein (arrowhead). Approximately 5 ul of an induced E.coli cell lysate expressing recombinant Cyclin L2 was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000 overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Human Small Intestine (formalin-fixed, paraffin-embedded) stained with CCNL2 antibody (GTX85686) at 5 μg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.