Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [8]
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Validation data
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- Product number
- NBP1-91678 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-91678, RRID:AB_11011344
- Product name
- Rabbit Polyclonal ARHGAP12 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human ARHGAP12 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Phosphoinositide 3-kinase enables phagocytosis of large particles by terminating actin assembly through Rac/Cdc42 GTPase-activating proteins.
Schlam D, Bagshaw RD, Freeman SA, Collins RF, Pawson T, Fairn GD, Grinstein S
Nature communications 2015 Oct 14;6:8623
Nature communications 2015 Oct 14;6:8623
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARHGAP12 Antibody [NBP1-91678] - Analysis in human cell line CAPAN-2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARHGAP12 Antibody [NBP1-91678] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARHGAP12 Antibody [NBP1-91678] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARHGAP12 Antibody [NBP1-91678] - Staining of human stomach shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARHGAP12 Antibody [NBP1-91678] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARHGAP12 Antibody [NBP1-91678] - Staining of human colorectal cancer shows strong cytoplasmic positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARHGAP12 Antibody [NBP1-91678] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARHGAP12 Antibody [NBP1-91678] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARHGAP12 Antibody [NBP1-91678] - Staining in human stomach and skeletal muscle tissues using NBP1-91678 antibody. Corresponding ARHGAP12 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARHGAP12 Antibody [NBP1-91678] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.