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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [7]
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- Product number
- TA590501 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal AMBRA1 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal AMBRA1 Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- AMBRA1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.05mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: AMBRA1 Antibody - Western blot analysis of AMBRA1 on brain extracts using TA590501 showing a band at approximately 132kDa
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMBRA1 (RC206255, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMBRA1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-AMBRA1 rabbit polyclonal antibody (TA590501).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation: AMBRA1 Antibody - IP analysis of AMBRA1 in various cell lysates.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: AMBRA1 Antibody - Immunohistochemical analysis of AMBRA1 on human cerebral cortex tissue
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-AMBRA1 rabbit polyclonal antibody. (TA590501)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AMBRA1 rabbit polyclonal antibody. (TA590501)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-AMBRA1 rabbit polyclonal antibody. (TA590501)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-AMBRA1 rabbit polyclonal antibody. (TA590501)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-AMBRA1 rabbit polyclonal antibody. (TA590501)
- Validation comment
- IHC
