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Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 12286-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP4F11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: HDKWQRLAS EGSARLDMFE HISLMTLDSL QKCVFSFESN CQEKPSEYIA AILELSAFVE KRNQQILLHT DFLYYLTPDG QRFRRACHLV HDFTDAVIQE RRRTLPTQGI DDFLKNKAKS KTLDFIDVLL LSKDEDGKEL SDEDIRAEAD TFMFEGHDTT ASGLSWVLYH LAKHPEYQEQ CRQEVQELLK DREPIEIEWD DLAQLPFLTM CIKESLRLHP PVPVISRCCT QDFVLPDGRV IPKGIVCLIN IIGIHYNPTV WPDPEVYDPF RFNQENIKER SPLAFIPFSA GPRNCIGQAF AMAEMKVVLA LTLLHFRILP THTEPRRKPE LILRAEGGLW LRVEPLGANS Q (175-524 aa encoded by BC016853)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.19 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human kidney tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed A549 cells using 12286-1-AP (CYP4F11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed A549 cells using 12286-1-AP (CYP4F11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using 12286-1-AP (CYP4F11 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using 12286-1-AP (CYP4F11 antibody) at dilution of 1:100 (under 40x lens).
