Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-57713 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX46 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KEGNEMEGEE LDPLDAYMEE VKEEVKKFNM RSVKGGGGNE KKSGPTVTKV VTVVTTKKAV VDSDKKKGEL MENDQDAM
- Concentration
- 0.1 mg/mL
Submitted references Seed sequence polymorphism rs2168518 and allele-specific target gene regulation of hsa-miR-4513.
Kiel C, Strunz T, Hasler D, Meister G, Grassmann F, Weber BHF
Human molecular genetics 2022 Mar 21;31(6):875-887
Human molecular genetics 2022 Mar 21;31(6):875-887
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DDX46 in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a DDX46 Polyclonal Antibody (Product # PA5-57713). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DDX46 using DDX46 Polyclonal Antibody (Product # PA5-57713) (A) shows similar pattern to an independent DDX46 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DDX46 in mouse cell line NIH-3T3 and rat cell line NBT-II using a DDX46 Polyclonal Antibody (Product # PA5-57713).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DDX46 in human testis tissue shows strong nuclear positivity in seminiferous tubules. Samples were probed using a DDX46 Polyclonal Antibody (Product # PA5-57713).