Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
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Validation data
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- Product number
- PA5-38811 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PERK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Liraglutide Treatment Reduces Endothelial Endoplasmic Reticulum Stress and Insulin Resistance in Patients With Diabetes Mellitus.
Bretón-Romero R, Weisbrod RM, Feng B, Holbrook M, Ko D, Stathos MM, Zhang JY, Fetterman JL, Hamburg NM
Journal of the American Heart Association 2018 Sep 18;7(18):e009379
Journal of the American Heart Association 2018 Sep 18;7(18):e009379
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF2AK3/PERK in extracts from HeLa cells and HepG2 cells using an EIF2AK3/PERK polyclonal antibody (Product # PA5-38811).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PERK Polyclonal Antibody (Product # PA5-38811) and a band at ~125 kDa corresponding to PERK was observed in all the cell lines tested and was increased upon Thapsigargin and Tunicamycin treatment in MCF7. Few uncharacterized bands between 40-50 kDa were also observed across the samples tested. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), MCF7 treated with Thapsigargin (1 µMol/L, 24hrs) (Lane 2), MCF7 treated with Tunicamycin (1 µg/mL, 24hrs), U-87 MG (Lane 4), HeLa (Lane 5), T-47D (Lane 6) and U-2 OS (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EIF2AK3/PERK in HepG2 cells using an EIF2AK3/PERK polyclonal antibody (Product # PA5-38811).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EIF2AK3/PERK in HepG2 cells using an EIF2AK3/PERK polyclonal antibody (Product # PA5-38811).