649401

antibody from BioLegend

Targeting: EIF2AK3 PEK, PERK

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

649401

Provider

BioLegend

Proper citation

BioLegend Cat#649401, RRID:AB_10642966

Product name

Purified anti-PERK Phospho (Ser713)

Antibody type

Polyclonal

Antigen

Modified synthetic peptide

Description

PERK (protein kinase-like endoplasmic reticulum kinase) is a type I membrane protein located in the endoplasmic reticulum (ER). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress. PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain. Phosphorylation of PERK at Ser713 can be used as a marker for its activation status.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µl

Storage

Upon receipt, store at -20°C.

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

293E cells were transfected with PERK-FLAG construct. Cell lysates were then immunoprecipitated with L5 antibody. Proteins were then treated with calf intestinal alkaline phosphatase (CIP). The proteins without CIP treatment were used as the control. Samples were separated by electrophoresis, transferred to nitrocellulose and probed with anti-PERK phospho (Ser713) antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminenescent system.

Conjugate

Unconjugated