649402

antibody from BioLegend

Targeting: EIF2AK3 PEK, PERK

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

649402

Provider

BioLegend

Proper citation

BioLegend Cat#649402, RRID:AB_10640071

Product name

Purified anti-PERK Phospho (Ser713)

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

Poly6494

Vial size

400 µL

Concentration

0.00 mg/mL

Storage

-20°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

293E cells were transfected with PERK-FLAG construct. Cell lysates were then immunoprecipitated with L5 antibody. Proteins were then treated with calf intestinal alkaline phosphatase (CIP). The proteins without CIP treatment were used as the control. Samples were separated by electrophoresis, transferred to nitrocellulose and probed with anti-PERK phospho (Ser713) antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminenescent system.

Conjugate

Unconjugated

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysate from 293T cells transfected with FLAG-PERK (lane 1) and FLAG-PERK S713A mutant (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-PERK Phospho (Ser713) antibody (clone Poly6494). Proteins were visualized using an HRP-conjugated anti-rabbit-IgG secondary and chemiluminescence detection.

Conjugate

Unconjugated