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- Antibody Data
- Antigen structure
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- Product number
- AF3999 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PERK Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human PERK in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9NZJ5- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation.
Characterization of a novel PERK kinase inhibitor with antitumor and antiangiogenic activity.
Polycystin-2 down-regulates cell proliferation via promoting PERK-dependent phosphorylation of eIF2alpha.
Xie W, Pariollaud M, Wixted WE, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames RS, Callahan J, Solari R, Sanchez Y, Diehl A, Li H
PloS one 2015;10(3):e0119738
PloS one 2015;10(3):e0119738
Characterization of a novel PERK kinase inhibitor with antitumor and antiangiogenic activity.
Atkins C, Liu Q, Minthorn E, Zhang SY, Figueroa DJ, Moss K, Stanley TB, Sanders B, Goetz A, Gaul N, Choudhry AE, Alsaid H, Jucker BM, Axten JM, Kumar R
Cancer research 2013 Mar 15;73(6):1993-2002
Cancer research 2013 Mar 15;73(6):1993-2002
Polycystin-2 down-regulates cell proliferation via promoting PERK-dependent phosphorylation of eIF2alpha.
Liang G, Yang J, Wang Z, Li Q, Tang Y, Chen XZ
Human molecular genetics 2008 Oct 15;17(20):3254-62
Human molecular genetics 2008 Oct 15;17(20):3254-62
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human PERK by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PERK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3999) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PERK at approximately 130 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human PERK Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PERK knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PERK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3999) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PERK at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
