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- Antigen structure
- References [12]
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- Product number
- NBP2-19127 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal ATG13 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is directed against the phosphorylated form of human ATG13 protein at the pS318 residue. NBP2-19127 was cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human ATG13 pS318 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human ATG13 is minimal by ELISA and western blot.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Fingolimod Augments Monomethylfumarate Killing of GBM Cells.
(Curcumin+sildenafil) enhances the efficacy of 5FU and anti-PD1 therapies in vivo.
The multi-kinase inhibitor lenvatinib interacts with the HDAC inhibitor entinostat to kill liver cancer cells.
GZ17-6.02 initiates DNA damage causing autophagosome-dependent HDAC degradation resulting in enhanced anti-PD1 checkpoint inhibitory antibody efficacy.
The identification and characterisation of autophagy inhibitors from the published kinase inhibitor sets.
Glutamine Metabolism Drives Growth in Advanced Hormone Receptor Positive Breast Cancer.
The Lethality of [Pazopanib + HDAC Inhibitors] Is Enhanced by Neratinib.
Fingolimod augments Pemetrexed killing of non-small cell lung cancer and overcomes resistance to ERBB inhibition.
Neratinib inhibits Hippo/YAP signaling, reduces mutant K-RAS expression, and kills pancreatic and blood cancer cells.
CRISPR screening using an expanded toolkit of autophagy reporters identifies TMEM41B as a novel autophagy factor.
The irreversible ERBB1/2/4 inhibitor neratinib interacts with the PARP1 inhibitor niraparib to kill ovarian cancer cells.
Hsp90-Cdc37 chaperone complex regulates Ulk1- and Atg13-mediated mitophagy.
Dent P, Booth L, Roberts JL, Poklepovic A, Hancock JF
Frontiers in oncology 2020;10:22
Frontiers in oncology 2020;10:22
(Curcumin+sildenafil) enhances the efficacy of 5FU and anti-PD1 therapies in vivo.
Dent P, Booth L, Roberts JL, Poklepovic A, Hancock JF
Journal of cellular physiology 2020 Oct;235(10):6862-6874
Journal of cellular physiology 2020 Oct;235(10):6862-6874
The multi-kinase inhibitor lenvatinib interacts with the HDAC inhibitor entinostat to kill liver cancer cells.
Roberts JL, Poklepovic A, Booth L, Dent P
Cellular signalling 2020 Jun;70:109573
Cellular signalling 2020 Jun;70:109573
GZ17-6.02 initiates DNA damage causing autophagosome-dependent HDAC degradation resulting in enhanced anti-PD1 checkpoint inhibitory antibody efficacy.
Booth L, Roberts JL, West C, Von Hoff D, Dent P
Journal of cellular physiology 2020 Jan 17;
Journal of cellular physiology 2020 Jan 17;
The identification and characterisation of autophagy inhibitors from the published kinase inhibitor sets.
Zachari M, Rainard JM, Pandarakalam GC, Robinson L, Gillespie J, Rajamanickam M, Hamon V, Morrison A, Ganley IG, McElroy SP
The Biochemical journal 2020 Feb 28;477(4):801-814
The Biochemical journal 2020 Feb 28;477(4):801-814
Glutamine Metabolism Drives Growth in Advanced Hormone Receptor Positive Breast Cancer.
Demas DM, Demo S, Fallah Y, Clarke R, Nephew KP, Althouse S, Sandusky G, He W, Shajahan-Haq AN
Frontiers in oncology 2019;9:686
Frontiers in oncology 2019;9:686
The Lethality of [Pazopanib + HDAC Inhibitors] Is Enhanced by Neratinib.
Booth L, Roberts JL, Poklepovic A, Dent P
Frontiers in oncology 2019;9:650
Frontiers in oncology 2019;9:650
Fingolimod augments Pemetrexed killing of non-small cell lung cancer and overcomes resistance to ERBB inhibition.
Booth L, Roberts JL, Spiegel S, Poklepovic A, Dent P
Cancer biology & therapy 2019;20(5):597-607
Cancer biology & therapy 2019;20(5):597-607
Neratinib inhibits Hippo/YAP signaling, reduces mutant K-RAS expression, and kills pancreatic and blood cancer cells.
Dent P, Booth L, Roberts JL, Liu J, Poklepovic A, Lalani AS, Tuveson D, Martinez J, Hancock JF
Oncogene 2019 Jul;38(30):5890-5904
Oncogene 2019 Jul;38(30):5890-5904
CRISPR screening using an expanded toolkit of autophagy reporters identifies TMEM41B as a novel autophagy factor.
Shoemaker CJ, Huang TQ, Weir NR, Polyakov NJ, Schultz SW, Denic V
PLoS biology 2019 Apr;17(4):e2007044
PLoS biology 2019 Apr;17(4):e2007044
The irreversible ERBB1/2/4 inhibitor neratinib interacts with the PARP1 inhibitor niraparib to kill ovarian cancer cells.
Booth L, Roberts JL, Samuel P, Avogadri-Connors F, Cutler RE, Lalani AS, Poklepovic A, Dent P
Cancer biology & therapy 2018 Jun 3;19(6):525-533
Cancer biology & therapy 2018 Jun 3;19(6):525-533
Hsp90-Cdc37 chaperone complex regulates Ulk1- and Atg13-mediated mitophagy.
Joo JH, Dorsey FC, Joshi A, Hennessy-Walters KM, Rose KL, McCastlain K, Zhang J, Iyengar R, Jung CH, Suen DF, Steeves MA, Yang CY, Prater SM, Kim DH, Thompson CB, Youle RJ, Ney PA, Cleveland JL, Kundu M
Molecular cell 2011 Aug 19;43(4):572-85
Molecular cell 2011 Aug 19;43(4):572-85
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATG13 [p Ser318] Antibody [NBP2-19127] - Antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13. In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blocking Buffer and probed with the primary antibody at 1ug/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer for 45 minutes at room temperature.
