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- References [35]
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- Product number
- 600-401-C49 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-C49, RRID:AB_11179920
- Product name
- Anti-ATG13 pS318 (RABBIT) Antibody - 600-401-C49
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references A Dual-Acting Nitric Oxide Donor and Phosphodiesterase 5 Inhibitor Activates Autophagy in Primary Skin Fibroblasts.
Dendritic autophagy degrades postsynaptic proteins and is required for long-term synaptic depression in mice.
GAK and PRKCD are positive regulators of PRKN-independent mitophagy.
TFG binds LC3C to regulate ULK1 localization and autophagosome formation.
GSK3B induces autophagy by phosphorylating ULK1.
Phosphoproteomic identification of ULK substrates reveals VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy.
Reducing FASN expression sensitizes acute myeloid leukemia cells to differentiation therapy.
The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability.
Design, Synthesis, and Characterization of an Orally Active Dual-Specific ULK1/2 Autophagy Inhibitor that Synergizes with the PARP Inhibitor Olaparib for the Treatment of Triple-Negative Breast Cancer.
Dissociation of nicotinic α7 and α4/β2 sub-receptor agonists for enhancing learning and attentional filtering in nonhuman primates.
Copper is an essential regulator of the autophagic kinases ULK1/2 to drive lung adenocarcinoma.
Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-Throughput Screening.
ULK2 is essential for degradation of ubiquitinated protein aggregates and homeostasis in skeletal muscle.
Negative regulation of autophagy by UBA6-BIRC6-mediated ubiquitination of LC3.
The kinase PERK and the transcription factor ATF4 play distinct and essential roles in autophagy resulting from tunicamycin-induced ER stress.
ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97.
Recycling endosomal CD133 functions as an inhibitor of autophagy at the pericentrosomal region.
Systematic analysis of ATG13 domain requirements for autophagy induction.
MAPK15 is part of the ULK complex and controls its activity to regulate early phases of the autophagic process.
SOX2 regulates common and specific stem cell features in the CNS and endoderm derived organs.
The ULK1-FBXW5-SEC23B nexus controls autophagy.
Phosphorylation of ULK1 affects autophagosome fusion and links chaperone-mediated autophagy to macroautophagy.
Study of ULK1 Catalytic Activity and Its Regulation.
Assessment of Posttranslational Modifications of ATG proteins.
Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator.
Stall in Canonical Autophagy-Lysosome Pathways Prompts Nucleophagy-Based Nuclear Breakdown in Neurodegeneration.
The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14.
Nutrient-regulated Phosphorylation of ATG13 Inhibits Starvation-induced Autophagy.
Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells.
Chaperone-like protein p32 regulates ULK1 stability and autophagy.
Small Molecule Inhibition of the Autophagy Kinase ULK1 and Identification of ULK1 Substrates.
Development of an HTS-Compatible Assay for the Discovery of Ulk1 Inhibitors.
CIP2A oncoprotein controls cell growth and autophagy through mTORC1 activation.
Autophagy control by the VEGF-C/NRP-2 axis in cancer and its implication for treatment resistance.
mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6.
Martínez-Martínez E, Atzei P, Vionnet C, Roubaty C, Kaeser-Pebernard S, Naef R, Dengjel J
International journal of molecular sciences 2022 Jun 20;23(12)
International journal of molecular sciences 2022 Jun 20;23(12)
Dendritic autophagy degrades postsynaptic proteins and is required for long-term synaptic depression in mice.
Kallergi E, Daskalaki AD, Kolaxi A, Camus C, Ioannou E, Mercaldo V, Haberkant P, Stein F, Sidiropoulou K, Dalezios Y, Savitski MM, Bagni C, Choquet D, Hosy E, Nikoletopoulou V
Nature communications 2022 Feb 3;13(1):680
Nature communications 2022 Feb 3;13(1):680
GAK and PRKCD are positive regulators of PRKN-independent mitophagy.
Munson MJ, Mathai BJ, Ng MYW, Trachsel-Moncho L, de la Ballina LR, Schultz SW, Aman Y, Lystad AH, Singh S, Singh S, Wesche J, Fang EF, Simonsen A
Nature communications 2021 Oct 20;12(1):6101
Nature communications 2021 Oct 20;12(1):6101
TFG binds LC3C to regulate ULK1 localization and autophagosome formation.
Carinci M, Testa B, Bordi M, Milletti G, Bonora M, Antonucci L, Ferraina C, Carro M, Kumar M, Ceglie D, Eck F, Nardacci R, le Guerroué F, Petrini S, Soriano ME, Caruana I, Doria V, Manifava M, Peron C, Lambrughi M, Tiranti V, Behrends C, Papaleo E, Pinton P, Giorgi C, Ktistakis NT, Locatelli F, Nazio F, Cecconi F
The EMBO journal 2021 May 17;40(10):e103563
The EMBO journal 2021 May 17;40(10):e103563
GSK3B induces autophagy by phosphorylating ULK1.
Ryu HY, Kim LE, Jeong H, Yeo BK, Lee JW, Nam H, Ha S, An HK, Park H, Jung S, Chung KM, Kim J, Lee BH, Cheong H, Kim EK, Yu SW
Experimental & molecular medicine 2021 Mar;53(3):369-383
Experimental & molecular medicine 2021 Mar;53(3):369-383
Phosphoproteomic identification of ULK substrates reveals VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy.
Mercer TJ, Ohashi Y, Boeing S, Jefferies HBJ, De Tito S, Flynn H, Tremel S, Zhang W, Wirth M, Frith D, Snijders AP, Williams RL, Tooze SA
The EMBO journal 2021 Jul 15;40(14):e105985
The EMBO journal 2021 Jul 15;40(14):e105985
Reducing FASN expression sensitizes acute myeloid leukemia cells to differentiation therapy.
Humbert M, Seiler K, Mosimann S, Rentsch V, Sharma K, Pandey AV, McKenna SL, Tschan MP
Cell death and differentiation 2021 Aug;28(8):2465-2481
Cell death and differentiation 2021 Aug;28(8):2465-2481
The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability.
Thayer JA, Awad O, Hegdekar N, Sarkar C, Tesfay H, Burt C, Zeng X, Feldman RA, Lipinski MM
Autophagy 2020 Jan;16(1):140-153
Autophagy 2020 Jan;16(1):140-153
Design, Synthesis, and Characterization of an Orally Active Dual-Specific ULK1/2 Autophagy Inhibitor that Synergizes with the PARP Inhibitor Olaparib for the Treatment of Triple-Negative Breast Cancer.
Ren H, Bakas NA, Vamos M, Chaikuad A, Limpert AS, Wimer CD, Brun SN, Lambert LJ, Tautz L, Celeridad M, Sheffler DJ, Knapp S, Shaw RJ, Cosford NDP
Journal of medicinal chemistry 2020 Dec 10;63(23):14609-14625
Journal of medicinal chemistry 2020 Dec 10;63(23):14609-14625
Dissociation of nicotinic α7 and α4/β2 sub-receptor agonists for enhancing learning and attentional filtering in nonhuman primates.
Azimi M, Oemisch M, Womelsdorf T
Psychopharmacology 2020 Apr;237(4):997-1010
Psychopharmacology 2020 Apr;237(4):997-1010
Copper is an essential regulator of the autophagic kinases ULK1/2 to drive lung adenocarcinoma.
Tsang T, Posimo JM, Gudiel AA, Cicchini M, Feldser DM, Brady DC
Nature cell biology 2020 Apr;22(4):412-424
Nature cell biology 2020 Apr;22(4):412-424
Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-Throughput Screening.
Wirth M, Tooze SA
Methods in molecular biology (Clifton, N.J.) 2019;1880:375-387
Methods in molecular biology (Clifton, N.J.) 2019;1880:375-387
ULK2 is essential for degradation of ubiquitinated protein aggregates and homeostasis in skeletal muscle.
Fuqua JD, Mere CP, Kronemberger A, Blomme J, Bae D, Turner KD, Harris MP, Scudese E, Edwards M, Ebert SM, de Sousa LGO, Bodine SC, Yang L, Adams CM, Lira VA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Nov;33(11):11735-11745
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Nov;33(11):11735-11745
Negative regulation of autophagy by UBA6-BIRC6-mediated ubiquitination of LC3.
Jia R, Bonifacino JS
eLife 2019 Nov 6;8
eLife 2019 Nov 6;8
The kinase PERK and the transcription factor ATF4 play distinct and essential roles in autophagy resulting from tunicamycin-induced ER stress.
Luhr M, Torgersen ML, Szalai P, Hashim A, Brech A, Staerk J, Engedal N
The Journal of biological chemistry 2019 May 17;294(20):8197-8217
The Journal of biological chemistry 2019 May 17;294(20):8197-8217
ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97.
Wang B, Maxwell BA, Joo JH, Gwon Y, Messing J, Mishra A, Shaw TI, Ward AL, Quan H, Sakurada SM, Pruett-Miller SM, Bertorini T, Vogel P, Kim HJ, Peng J, Taylor JP, Kundu M
Molecular cell 2019 May 16;74(4):742-757.e8
Molecular cell 2019 May 16;74(4):742-757.e8
Recycling endosomal CD133 functions as an inhibitor of autophagy at the pericentrosomal region.
Izumi H, Li Y, Shibaki M, Mori D, Yasunami M, Sato S, Matsunaga H, Mae T, Kodama K, Kamijo T, Kaneko Y, Nakagawara A
Scientific reports 2019 Feb 19;9(1):2236
Scientific reports 2019 Feb 19;9(1):2236
Systematic analysis of ATG13 domain requirements for autophagy induction.
Wallot-Hieke N, Verma N, Schlütermann D, Berleth N, Deitersen J, Böhler P, Stuhldreier F, Wu W, Seggewiß S, Peter C, Gohlke H, Mizushima N, Stork B
Autophagy 2018;14(5):743-763
Autophagy 2018;14(5):743-763
MAPK15 is part of the ULK complex and controls its activity to regulate early phases of the autophagic process.
Colecchia D, Dapporto F, Tronnolone S, Salvini L, Chiariello M
The Journal of biological chemistry 2018 Oct 12;293(41):15962-15976
The Journal of biological chemistry 2018 Oct 12;293(41):15962-15976
SOX2 regulates common and specific stem cell features in the CNS and endoderm derived organs.
Hagey DW, Klum S, Kurtsdotter I, Zaouter C, Topcic D, Andersson O, Bergsland M, Muhr J
PLoS genetics 2018 Feb;14(2):e1007224
PLoS genetics 2018 Feb;14(2):e1007224
The ULK1-FBXW5-SEC23B nexus controls autophagy.
Jeong YT, Simoneschi D, Keegan S, Melville D, Adler NS, Saraf A, Florens L, Washburn MP, Cavasotto CN, Fenyö D, Cuervo AM, Rossi M, Pagano M
eLife 2018 Dec 31;7
eLife 2018 Dec 31;7
Phosphorylation of ULK1 affects autophagosome fusion and links chaperone-mediated autophagy to macroautophagy.
Wang C, Wang H, Zhang D, Luo W, Liu R, Xu D, Diao L, Liao L, Liu Z
Nature communications 2018 Aug 28;9(1):3492
Nature communications 2018 Aug 28;9(1):3492
Study of ULK1 Catalytic Activity and Its Regulation.
Stork B, Dengjel J
Methods in enzymology 2017;587:391-404
Methods in enzymology 2017;587:391-404
Assessment of Posttranslational Modifications of ATG proteins.
Xie Y, Kang R, Tang D
Methods in enzymology 2017;587:171-188
Methods in enzymology 2017;587:171-188
Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator.
Jung J, Nayak A, Schaeffer V, Starzetz T, Kirsch AK, Müller S, Dikic I, Mittelbronn M, Behrends C
eLife 2017 Feb 14;6
eLife 2017 Feb 14;6
Stall in Canonical Autophagy-Lysosome Pathways Prompts Nucleophagy-Based Nuclear Breakdown in Neurodegeneration.
Baron O, Boudi A, Dias C, Schilling M, Nölle A, Vizcay-Barrena G, Rattray I, Jungbluth H, Scheper W, Fleck RA, Bates GP, Fanto M
Current biology : CB 2017 Dec 4;27(23):3626-3642.e6
Current biology : CB 2017 Dec 4;27(23):3626-3642.e6
The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14.
Park JM, Jung CH, Seo M, Otto NM, Grunwald D, Kim KH, Moriarity B, Kim YM, Starker C, Nho RS, Voytas D, Kim DH
Autophagy 2016;12(3):547-64
Autophagy 2016;12(3):547-64
Nutrient-regulated Phosphorylation of ATG13 Inhibits Starvation-induced Autophagy.
Puente C, Hendrickson RC, Jiang X
The Journal of biological chemistry 2016 Mar 11;291(11):6026-6035
The Journal of biological chemistry 2016 Mar 11;291(11):6026-6035
Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells.
Hieke N, Löffler AS, Kaizuka T, Berleth N, Böhler P, Drießen S, Stuhldreier F, Friesen O, Assani K, Schmitz K, Peter C, Diedrich B, Dengjel J, Holland P, Simonsen A, Wesselborg S, Mizushima N, Stork B
Autophagy 2015;11(9):1471-83
Autophagy 2015;11(9):1471-83
Chaperone-like protein p32 regulates ULK1 stability and autophagy.
Jiao H, Su GQ, Dong W, Zhang L, Xie W, Yao LM, Chen P, Wang ZX, Liou YC, You H
Cell death and differentiation 2015 Nov;22(11):1812-23
Cell death and differentiation 2015 Nov;22(11):1812-23
Small Molecule Inhibition of the Autophagy Kinase ULK1 and Identification of ULK1 Substrates.
Egan DF, Chun MG, Vamos M, Zou H, Rong J, Miller CJ, Lou HJ, Raveendra-Panickar D, Yang CC, Sheffler DJ, Teriete P, Asara JM, Turk BE, Cosford ND, Shaw RJ
Molecular cell 2015 Jul 16;59(2):285-97
Molecular cell 2015 Jul 16;59(2):285-97
Development of an HTS-Compatible Assay for the Discovery of Ulk1 Inhibitors.
Rosenberg LH, Lafitte M, Grant W, Chen W, Cleveland JL, Duckett DR
Journal of biomolecular screening 2015 Aug;20(7):913-20
Journal of biomolecular screening 2015 Aug;20(7):913-20
CIP2A oncoprotein controls cell growth and autophagy through mTORC1 activation.
Puustinen P, Rytter A, Mortensen M, Kohonen P, Moreira JM, Jäättelä M
The Journal of cell biology 2014 Mar 3;204(5):713-27
The Journal of cell biology 2014 Mar 3;204(5):713-27
Autophagy control by the VEGF-C/NRP-2 axis in cancer and its implication for treatment resistance.
Stanton MJ, Dutta S, Zhang H, Polavaram NS, Leontovich AA, Hönscheid P, Sinicrope FA, Tindall DJ, Muders MH, Datta K
Cancer research 2013 Jan 1;73(1):160-71
Cancer research 2013 Jan 1;73(1):160-71
mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6.
Nazio F, Strappazzon F, Antonioli M, Bielli P, Cianfanelli V, Bordi M, Gretzmeier C, Dengjel J, Piacentini M, Fimia GM, Cecconi F
Nature cell biology 2013 Apr;15(4):406-16
Nature cell biology 2013 Apr;15(4):406-16
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-ATG13 pS318 antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13). In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto (p/n B501-0500) and probed with the primary antibody at 1µg/mL overnight at 4°C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer (p/n MB-070) for 45 minutes at room temperature. In collaboration with Charles Dorsey at Eli Lilly, Indianapolis, IN and John Cleveland at Scripps, Jupiter, FL.
- Validation comment
- Western Blot
