PA5-59150

antibody from Invitrogen Antibodies

Targeting: CKAP5 ch-TOG, KIAA0097, TOG, TOGp

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-59150

Provider

Invitrogen Antibodies

Product name

CKAP5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MSSKLNQARS MSGHPEAAQM VRREFQLDLD EIENDNGTVR CEMPELVQHK LDDIFEPVLI PEPKIRAVSP HFDDMHSNTA STINFI

Concentration

0.2 mg/mL

References

Submitted references

Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization.

Ryan EL, Shelford J, Massam-Wu T, Bayliss R, Royle SJ
Journal of cell science 2021 Feb 1;134(3)

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Human Cytomegalovirus Exploits TACC3 To Control Microtubule Dynamics and Late Stages of Infection.

Furey C, Astar H, Walsh D
Journal of virology 2021 Aug 25;95(18):e0082121

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of CKAP5 in human cell line U-2 OS shows positivity in nucleoli, plasma membrane & cytoplasm. Samples were probed using a CKAP5 Polyclonal Antibody (Product # PA5-59150).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of CKAP5 in human cell line U-2 OS using CKAP5 Polyclonal Antibody (Product # PA5-59150) shows localization to nucleoli, plasma membrane & centrosome.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CKAP5 in human cerebellum using CKAP5 Polyclonal Antibody (Product # PA5-59150) shows moderate cytoplasmic positivity in Purkinje cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CKAP5 in human cerebral cortex using CKAP5 Polyclonal Antibody (Product # PA5-59150) shows strong cytoplasmic positivity in neurons.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CKAP5 in human testis using CKAP5 Polyclonal Antibody (Product # PA5-59150) shows strong cytoplasmic positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CKAP5 in human placenta using CKAP5 Polyclonal Antibody (Product # PA5-59150) shows strong cytoplasmic positivity in trophoblastic cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3. Co-rerouting of endogenous complex members following knocksideways in knock-in cell lines. (A-D) Knocksideways experiments using each knock-in cell line expressing dark MitoTrap. (A) CLTA-FKBP-GFP cells. (B) GFP-FKBP-TACC3 cells. (C) chTOG-FKBP-GFP cells. (D) GTSE1-FKBP-GFP cells. Representative confocal micrographs of cells that were either untreated or treated with rapamycin (200 nM) for 30 min, fixed and stained for tubulin and either CHC, TACC3, chTOG or GTSE1 (protein of interest, POI; red). Scale bars: 10 um. Right, quantification of images. Spindle localization of the target protein ( x -axis) and the POI ( y -axis) in control (red) and knocksideways (turquoise) cells. Spindle localization is the ratio of spindle to cytoplasmic fluorescence shown on a log 2 scale (where a value of 1 is twice the amount of fluorescence signal in the spindle regions of interest as in the cytoplasmic regions of interest, and -1 indicates half the amount of fluorescence in the spindle regions of interest versus in the cytoplasmic regions of interest). Quantification of cells from three or more experiments is shown.