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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- M05324 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ch TOG/CKAP5 Antibody Picoband™ (monoclonal, 3C13)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for ch TOG/CKAP5 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3C13
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Ch TOG/CKAP5 using anti-Ch TOG/CKAP5 antibody (M05324). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Ch TOG/CKAP5 antigen affinity purified monoclonal antibody (Catalog # M05324) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Ch TOG/CKAP5 at approximately 225KD. The expected band size for Ch TOG/CKAP5 is at 225KD.
- Additional image
