Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 620401 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#620401, RRID:AB_2080729
- Product name
- Purified anti-ch-TOG
- Antibody type
- Polyclonal
- Antigen
- Partial recombinant protein, containing approximately 200 amino acids from the C-terminus
- Description
- ch-TOG (also known as colonic and hepatic tumor over-expressed protein) is a 218 kD member of the TOG/XMAP215 family containing heat repeats. This protein is localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis. Ch-TOG is believed to function in spindle microtubule organization and has been shown to interact with TACC1, TACC3,and tubulin. The Poly6204 antibody has be shown to be useful for Western blotting of the human ch-TOG protein.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 50 µl
- Storage
- Upon receipt, store frozen at -20° C.
Submitted references Antimitotic effect of the retinoid 4-oxo-fenretinide through inhibition of tubulin polymerization: a novel mechanism of retinoid growth-inhibitory activity.
Appierto V, Tiberio P, Cavadini E, Casalini P, Cappelletti G, Formelli F
Molecular cancer therapeutics 2009 Dec;8(12):3360-8
Molecular cancer therapeutics 2009 Dec;8(12):3360-8
No comments: Submit comment
Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with polyclonal antibody against ch-TOG. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated
