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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- NBP1-06513 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-06513, RRID:AB_1582342
- Product name
- Rabbit Polyclonal ch-TOG Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references In vivo mitotic spindle scaling can be modulated by changing the levels of a single protein: the microtubule polymerase XMAP215.
Milunovic-Jevtic A, Jevtic P, Levy DL, Gatlin JC
Molecular biology of the cell 2018 Jun 1;29(11):1311-1317
Molecular biology of the cell 2018 Jun 1;29(11):1311-1317
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: ch-TOG Antibody [NBP1-06513] - Western Blot and Immunoprecipitation: ch-TOG antibody [NBP1-06513]- Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ch-TOG antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ch-TOG was also immunoprecipitated by rabbit anti-ch-TOG antibodies NBP1-06511and NBP1-06512, which recognize upstream epitopes.
