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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- NBP2-22407 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Ankyrin Brain Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx. >200kDa. No cross-reactivity against Ankyrin-G
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ankyrin Brain Antibody (S105-17) [NBP2-22407] - analysis of Rat brain membrane lysate showing detection of Ankyrin B protein using Mouse Anti-Ankyrin B Monoclonal Antibody, Clone S105-17 . Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-17) [NBP2-22407] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin-B Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.
