- HPA029524 - Atlas Antibodies SEPTIN7 antibody

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
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Experimental details: Western blot analysis using Anti-SEPT7 antibody HPA029524 (A) shows similar pattern to independent antibody HPA023309 (B).
Antibody #2 product nr: HPA023309
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Sample type: MOUSE, RAT

Supportive validation

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, plasma membrane & actin filaments.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using HPA029524 antibody. Corresponding SEPT7 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex, lymph node, skeletal muscle and testis using Anti-SEPT7 antibody HPA029524 (A) shows similar protein distribution across tissues to independent antibody HPA023309 (B).
Antibody #2 product nr: HPA023309
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human cerebral cortex shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human skeletal muscle shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human testis shows moderate positivity in cells in seminiferous ducts.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in non-germinal center cells.
Sample type: HUMAN

HPA029524