13818-1-AP

antibody from Invitrogen Antibodies

Targeting: SEPTIN7 CDC10, CDC3, SEPT7, SEPT7A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

13818-1-AP

Provider

Invitrogen Antibodies

Product name

SEPT7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: VIDYIDSKF EDYLNAESRV NRRQMPDNRV QCCLYFIAPS GHGLKPLDIE FMKRLHEKVN IIPLIAKADT LTPEECQQFK KQIMKEIQEH KIKIYEFPET DDEEENKLVK KIKDRLPLAV VGSNTIIEVN GKRVRGRQYP WGVAEVENGE HCDFTILRNM LIRTHMQDLK DVTNNVHYEN YRSRKLAAVT YNGVDNNKNK GQLTKSPLAQ MEEERREHVA KMKKMEMEME QVFEMKVKEK VQKLKDSEAE LQRRHEQMKK NLEAQHKELE EKRRQFEDEK ANWEAQQRIL EQQNSSRTLE KNKKKGKIF (129-437 aa encoded by BC025987)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 13818-1-AP (SEPT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 13818-1-AP (SEPT7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat testis tissue were subjected to SDS PAGE followed by western blot with 13818-1-AP (SEPT7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13818-1-AP (SEPT7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 13818-1-AP (SEPT7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using SEPT7 antibody 13818-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF result of anti-SEPT7 (13818-1-AP, 1:1000) with hTERT-RPE cell by Dr. Moshe Kim. Green: SEPT7. Blue: DNA. 1% PFA fixed.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13818-1-AP (SEPT7 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13818-1-AP (SEPT7 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 13818-1-AP (SEPT7 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 13818-1-AP (SEPT7 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13818-1-AP (SEPT7 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13818-1-AP (SEPT7 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).