Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
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Validation data
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- Product number
- 13818-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEPT7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 13818-1-AP (SEPT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 13818-1-AP (SEPT7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Rat testis tissue were subjected to SDS PAGE followed by western blot with 13818-1-AP (SEPT7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13818-1-AP (SEPT7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 13818-1-AP (SEPT7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of HepG2 cells, using SEPT7 antibody 13818-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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- Invitrogen Antibodies (provider)
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- Experimental details
- IF result of anti-SEPT7 (13818-1-AP, 1:1000) with hTERT-RPE cell by Dr. Moshe Kim. Green: SEPT7. Blue: DNA. 1% PFA fixed.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13818-1-AP (SEPT7 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 13818-1-AP (SEPT7 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 13818-1-AP (SEPT7 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 13818-1-AP (SEPT7 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13818-1-AP (SEPT7 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13818-1-AP (SEPT7 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).