NBP1-85731

antibody from Novus Biologicals

Targeting: SEPTIN7 CDC10, CDC3, SEPT7, SEPT7A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-85731

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-85731, RRID:AB_11040844

Product name

Rabbit Polyclonal Septin-7 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, mouse, rat Septin-7 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Septin-7 Antibody [NBP1-85731] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Septin-7 Antibody [NBP1-85731] - Analysis in human cell line U-2 OS.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Septin-7 Antibody [NBP1-85731] - Analysis using Anti-SEPT7 antibody NBP1-85731 (A) shows similar pattern to independent antibody NBP1-85730 (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human lymph node shows strong cytoplasmic positivity in non - germinal center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human cerebral cortex, lymph node, skeletal muscle and testis using Anti-SEPT7 antibody NBP1-85731 (A) shows similar protein distribution across tissues to independent antibody NBP1-85730 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human testis shows moderate positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human lymph node shows moderate cytoplasmic positivity in non-germinal center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-7 Antibody [NBP1-85731] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.