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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- TA319490 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-Tamalin antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-Tamalin antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- Grasp
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.03 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-Tamalin to detect over-expressed Tamalin in HEK293 cells (lane 2, arrowhead). Lane 1 shows the non-transfected control. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:2,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Mouse brain lysate was immunoprecipitated with anti-Tamalin antiserum. A blot was prepared and probed with affinity purified anti-Tamalin. Lane 1 is wild-type brain lysate; Lane 2 is Tamalin knock-out brain lysate. The membrane was probed with the primary antibody at a 1:1,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
- Validation comment
- WB
