Explore
Validate
Learn
Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX48721 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48721, RRID:AB_11162576
- Product name
- GRASP antibody
- Antibody type
- Polyclonal
- Reactivity
- Mouse
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's affinity purified anti-Tamalin to detect over-expressed Tamalin in HEK293 cells (lane 2, arrowhead). Lane 1 shows the non-transfected control. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:2,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's affinity purified anti-Tamalin to detect over-expressed Tamalin in HEK293 cells (lane 2, arrowhead). Lane 1 shows the non-transfected control. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:2,000 dilution.
Enhanced validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Mouse brain lysate was immunoprecipitated with anti-Tamalin antiserum. A blot was prepared and probed with affinity purified anti-Tamalin. Lane 1 is wild-type brain lysate; Lane 2 is Tamalin knock-out brain lysate. The membrane was probed with the primary antibody at a 1:1,000 dilution.
